Bacterial CellCultures in a Lab-on-a-Disc: a Simple and Versatile Machine for Quantification of Antibiotic Treatment Efficacy
Pathogenic bacterial biofilms might be life threatening, tremendously decrease affected individual’s top quality of life and are a substantial burden on the healthcare system. Current methods for evaluation of antibacterial therapies in clinics and in vitro strategies utilized in drug development and screening each do not facilitate biofilm formation or are cumbersome to operate, need huge reagent volumes and are costly, limiting their usability.
To deal with these factors, this work presents the occasion of a sturdy in vitro cell custom platform appropriate with confocal microscopy.
The platform shaped as a compact disc, facilitates long term bacterial custom with out exterior pumps and tubing and might be operated for numerous days with out further liquid coping with. As an example, Pseudomonas aeruginosa biofilm is grown from single cells and it is confirmed that:
1) the platform delivers reproducible and reliable outcomes; 2) progress depends on motion cost and progress medium composition; and three) effi-cacy of antibiotic treatment will depend upon the formed biofilm. This platform permits biofilm progress, quantification and treatment as in a conventional motion setup, whereas decreasing the equipment barrier of lab-on-chip strategies. It offers an easy-to-use, moderately priced risk for end clients working with cell culturing in relation to e.g. diagnostics and drug screening.
Propofol Impacts Non-Small-Cell Lung Most cancers Cell Biology By Regulating the miR-21/PTEN/AKT Pathway In Vitro and In Vivo
Background: Propofol is a standard sedative-hypnotic drug historically used for inducing and sustaining basic anesthesia. Current research have drawn consideration to the nonanesthetic results of propofol, however the potential mechanism by which propofol suppresses non-small-cell lung most cancers (NSCLC) development has not been totally elucidated.
Strategies: For the in vitro experiments, we used propofol (0, 2, 5, and 10 µg/mL) to deal with A549 cells for 1, 4, and 12 hours and Cell Counting Equipment-8 (CCK-8) to detect proliferation. Apoptosis was measured with circulation cytometry. We additionally transfected A549 cells with an microribonucleic acid-21 (miR-21) mimic or detrimental management ribonucleic acid (RNA) duplex and phosphatase and tensin homolog, deleted on chromosome 10 (PTEN) small interfering ribonucleic acid (siRNA) or detrimental management. PTEN, phosphorylated protein kinase B (pAKT), and protein kinase B (AKT) expression had been detected utilizing Western blotting, whereas miR-21 expression was examined by real-time polymerase chain response (RT-PCR).
In vivo, nude mice got injections of A549 cells to develop xenograft tumors; Eight days later, the mice had been intraperitoneally injected with propofol (35 mg/kg) or soybean oil. Tumors had been then collected from mice and analyzed by immunohistochemistry and Western blotting.
Outcomes: Propofol inhibited development (1 hour, P = .001; Four hours, P ≤ .0001; 12 hours, P = .0004) and miR-21 expression (P ≤ .0001) and induced apoptosis (1 hour, P = .0022; Four hours, P = .0005; 12 hours, P ≤ .0001) in A549 cells in a time and concentration-dependent method. MiR-21 mimic and PTEN siRNA transfection antagonized the suppressive results of propofol on A549 cells by reducing PTEN protein
expression (imply variations [MD] [95% confidence interval {CI}], -0.51 [-0.86 to 0.16], P = .0058; MD [95% CI], 0.81 [0.07-1.55], P = .0349, respectively), leading to a rise in pAKT ranges (MD [95% CI] = -0.82 [-1.46 to -0.18], P = .0133) following propofol publicity. In vivo, propofol therapy diminished NSCLC tumor development (MD [95% CI] = -109.47 [-167.03 to -51.91], P ≤ .0001) and promoted apoptosis (MD [95% CI] = 38.53 [11.69-65.36], P = .0093).
Conclusions: Our examine indicated that propofol inhibited A549 cell development, accelerated apoptosis through the miR-21/PTEN/AKT pathway in vitro, suppressed NSCLC tumor cell development, and promoted apoptosis in vivo. Our findings present new implications for propofol in most cancers remedy and point out that propofol is extraordinarily advantageous in surgical therapy.
RFP Control Lentivirus (Puro) |
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| LV096 | ABM | 4 x 500 ul | EUR 195 |
CMV control lentivirus (Hygro) |
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| CMV-Null-Hygro | GenTarget | 1 x107 IFU/ml x 200ul | EUR 206.5 |
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Description: Negative control lentivirus contains a null spacer insert under CMV promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter. |
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CAR negative control: CD28-CD3ζ (GFP-Puro) Lentivirus |
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| CAR-ctr8 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 276.5 |
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Description: CAR (CD28) control lentivirus without targeting domain, containing GFP-Puromycin dual selection |
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EF1a control lentivirus (Hygro) |
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| EF1a-Null-Hygro | GenTarget | 1 x107 IFU/ml x 200ul | EUR 206.5 |
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Description: Negative control lentivirus contains a null spacer insert under EF1a promoter, serves as the negative control of lentivurs treatment for the specificity of any target expression effects. It has the hygromycin selection under RSV promoter. |
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RFP (mRuby3) Control Lentivirus |
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| LV101 | ABM | 4 x 500 ul | EUR 195 |
Scrambled shRNA Control Lentivirus |
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| LSV-0024-1S | LipExoGen | 2x10^6 | EUR 395 |
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Description: vsv-g |
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Scrambled shRNA Control Lentivirus |
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| LSV-0024-2S | LipExoGen | 2x10^6 | EUR 395 |
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Description: vsv-g |
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Scrambled shRNA Control Lentivirus |
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| LSV-0024-3S | LipExoGen | 2x10^6 | EUR 395 |
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Description: vsv-g |
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Scrambled shRNA Control Lentivirus |
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| LSV-0024-4S | LipExoGen | 2x10^6 | EUR 395 |
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Description: vsv-g |
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Scrambled shRNA Control Lentivirus |
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| LSV-0024-5S | LipExoGen | 2x10^6 | EUR 395 |
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Description: vsv-g |
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Scrambled shRNA Control Lentivirus |
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| LSV-0024-6S | LipExoGen | 2x10^6 | EUR 395 |
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Description: vsv-g |
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Scrambled shRNA Control Lentivirus |
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| LSV-0024-7S | LipExoGen | 2.5x10^6 | EUR 395 |
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Description: vsv-g |
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Scrambled shRNA Control Lentivirus |
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| LSV-0024-8S | LipExoGen | 2.5x10^6 | EUR 395 |
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Description: vsv-g |
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CAR negative control: 4-1BB-CD3ζ (GFP-Puro) Lentivirus |
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| CAR-ctr7 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 276.5 |
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Description: CAR (4-1BB) control lentivirus without targeting domain, containing GFP-Puromycin dual selection |
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Negative Control Luciferase Lentivirus |
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| 79578 | BPS Bioscience | 500 µl x 2 | EUR 860 |
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Description: The Negative Control Lentivirus (Firefly Luciferase) are replication incompetent, HIV-based, VSV-G pseudo typed lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The particles contain a firefly luciferase gene under the control of a minimal TATA promoter, without any additional transcriptional response elements. |
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RFP (TagRFP-T) Control Lentivirus |
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| LV097 | ABM | 4 x 500 ul | EUR 195 |
CMV control lentivirus (No Selection) |
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| CMV-Null- | GenTarget | 1 x10^7 IFU/ml x 200ul | EUR 206.5 |
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Description: Negative control lentivirus under CMV promoter, does not contain any antibiotic selection. |
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Expression Negative Control Lentivirus (G418) |
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| 79902-G | BPS Bioscience | 500 µl x 2 | EUR 795 |
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Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_ |
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Expression Negative Control Lentivirus (Puromycin) |
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| 79902-P | BPS Bioscience | 500 µl x 2 | EUR 795 |
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Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_ |
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Expression Negative Control Lentivirus (Hygromycin) |
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| 79902-H | BPS Bioscience | 500 µl x 2 | EUR 795 |
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Description: The Expression Negative Control Lentivirus are replication incompetent, HIV-based, VSV-G pseudotyped lentiviral particles that are ready to be transduced into almost all types of mammalian cells, including primary and non-dividing cells. The controls package the same virus particles as the target expression virus, but they do not express a specific protein under the CMV promoter. _x000D_The Expression Negative Control Lentivirus (G418) expresses the gene for aminoglycoside 3' phosphotransferase, which confers resistance to kanamycin, neomycin, and geneticin (G418)._x000D_The Expression Negative Control Lentivirus (Hygromycin) expresses the gene for hygromycin B phosphotransferase, which confers resistance to Hygromycin._x000D_The Expression Negative Control Lentivirus (Puromycin) expresses the gene for puromycin N-acetyl-transferase, which confers resistance to puromycin._x000D_ _x000D_ |
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CAR negative control: CD28-CD3ζ (No Select) Lentivirus |
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| CAR-ctr6 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 276.5 |
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Description: CAR (CD28) control lentivirus without targeting domain, does not contain any antibiotic selection |
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CMV Control (No Antibiotic), Concentrated Lentivirus in PBS |
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| CMV-Null-PBS | GenTarget | 1 x10^8 IFU/ml x 200ul | EUR 455 |
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Description: Concentrated Negative control lentivirus under CMV promoter, contains no any antibioic selection. |
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EF1a Control (No Antibiotic), Concentrated Lentivirus in PBS |
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| EF1a-Null-PBS | GenTarget | 1 x10^8 IFU/ml x 200ul | EUR 455 |
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Description: Concentrated Negative control lentivirus under EF1a promoter, contains no any antibioic selection. |
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CAR negative control: 4-1BB-CD3ζ (No Select) Lentivirus |
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| CAR-ctr5 | GenTarget | 1x10^8 IFU/ml x 200ul | EUR 276.5 |
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Description: CAR (4-1BB) control lentivirus without targeting domain, do not any antibiotic selection |
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AR-RFP (GFP) Lentivirus |
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| LVP913-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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AR-Luc (GFP) Lentivirus |
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| LVP914-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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G_RE-RFP (GFP) Lentivirus |
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| LVP950-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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G_RE-Luc (GFP) Lentivirus |
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| LVP951-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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H_RE-RFP (GFP) Lentivirus |
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| LVP970-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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H_RE-Luc (GFP) Lentivirus |
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| LVP971-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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ER-RFP (GFP) Lentivirus |
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| LVP974-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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ER-Luc (GFP) Lentivirus |
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| LVP975-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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MB-RFP (GFP) Lentivirus |
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| LVP1022-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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MB-Luc (GFP) Lentivirus |
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| LVP1023-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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AR-Rluc (GFP) Lentivirus |
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| LVP915-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under the minimal promoter contains 4 tandem repeats of Androgen Response Element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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Gli-RFP (GFP) Lentivirus |
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| LVP946-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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Gli-Luc (GFP) Lentivirus |
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| LVP947-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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G_RE-Rluc (GFP) Lentivirus |
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| LVP952-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embedded 4 tandem repeats of glucocorticoid response element (G-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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AP1-RFP (GFP) Lentivirus |
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| LVP954-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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AP1-Luc (GFP) Lentivirus |
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| LVP955-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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SRE-RFP (GFP) Lentivirus |
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| LVP958-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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SRE-Luc (GFP) Lentivirus |
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| LVP959-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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RBP_JK-RFP (GFP) Lentivirus |
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| LVP962-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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RBP_JK-Luc (GFP) Lentivirus |
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| LVP963-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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H_RE-Rluc (GFP) Lentivirus |
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| LVP972-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 6 tandem repeats of hypoxia transcriptional response element (H-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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ER-Rluc (GFP) Lentivirus |
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| LVP976-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of estrogen receptor response element (ER-RE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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P53-RFP (GFP) Lentivirus |
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| LVP978-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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P53-Luc (GFP) Lentivirus |
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| LVP979-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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ARE-RFP (GFP) Lentivirus |
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| LVP982-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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ARE-Luc (GFP) Lentivirus |
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| LVP983-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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B29-RFP (GFP) Lentivirus |
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| LVP994-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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B29-Luc (GFP) Lentivirus |
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| LVP995-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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MB-Rluc (GFP) Lentivirus |
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| LVP1024-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under human Myoglobin 's promoter which only expressed in muscle, predominantly in cardiac and skeletal myocytes. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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SPB-RFP (GFP) Lentivirus |
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| LVP1026-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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SPB-Luc (GFP) Lentivirus |
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| LVP1027-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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aFP-RFP (GFP) Lentivirus |
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| LVP1034-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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aFP-Luc (GFP) Lentivirus |
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| LVP1035-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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HE4-RFP (GFP) Lentivirus |
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| LVP1042-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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HE4-Luc (GFP) Lentivirus |
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| LVP1043-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under human HE4's promoter which is overexpressed in ovarian cancer cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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PSA-RFP (GFP) Lentivirus |
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| LVP1046-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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PSA-Luc (GFP) Lentivirus |
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| LVP1047-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under human PSA's promoter which expressed in normal prostate epithelium and prostate cancers. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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ISRE-RFP (GFP) Lentivirus |
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| LVP938-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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ISRE-Luc (GFP) Lentivirus |
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| LVP939-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter undera minimal promoter embeded 8 tandem repeats of ISRE (Interferon Stimulated Response Element). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CRE_TRE-RFP (GFP) Lentivirus |
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| LVP942-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CRE_TRE-Luc (GFP) Lentivirus |
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| LVP943-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter embeded 8 tandem repeats of cAMP response motif (CRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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Gli-Rluc (GFP) Lentivirus |
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| LVP948-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter embeded 8 tandem repeats of Gli responsive element (hedgehog pathway). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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AP1-Rluc (GFP) Lentivirus |
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| LVP956-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 8 tandem repeats of AP1 Responsive Element (AP1-RE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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SRE-Rluc (GFP) Lentivirus |
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| LVP960-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of serum response element (SRE) . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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RBP_JK-Rluc (GFP) Lentivirus |
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| LVP964-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of RBP-JK transcriptional responsive element (RBP-JK TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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NFkB-RFP (GFP) Lentivirus |
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| LVP966-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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NFkB-Luc (GFP) Lentivirus |
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| LVP967-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 4 tandem repeats of NFκB transcriptional response element (NFkB-TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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P53-Rluc (GFP) Lentivirus |
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| LVP980-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded with optimized tandem repeats of a few most potent P53 transcriptional response element. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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ARE-Rluc (GFP) Lentivirus |
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| LVP984-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under a minimal promoter that embedded 4 tandem repeats of antioxidant response element (ARE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CEBP-RFP (GFP) Lentivirus |
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| LVP986-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CEBP-Luc (GFP) Lentivirus |
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| LVP987-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under a minimal promoter that embedded 8 tandem repeats of C/EBP transcriptional response element (C/EBP-TRE). This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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B29-Rluc (GFP) Lentivirus |
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| LVP996-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under a human Immunoglobulin gene's promoter which demonstrates B-cell specific expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CD14-RFP (GFP) Lentivirus |
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| LVP998-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CD14-Luc (GFP) Lentivirus |
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| LVP999-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD14 promoter which demonstrates strongly upregulated expression during monocytic cell differentiation. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CD43-RFP (GFP) Lentivirus |
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| LVP1002-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CD43-Luc (GFP) Lentivirus |
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| LVP1003-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD43 promoter which expressed on the surface of leukocytes and platelets.. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CD45-RFP (GFP) Lentivirus |
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| LVP1006-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CD45-Luc (GFP) Lentivirus |
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| LVP1007-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD45's promoter which expressed exclusively by all hematopoietic cells except erythrocytes and platelets. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CD68-RFP (GFP) Lentivirus |
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| LVP1010-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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CD68-Luc (GFP) Lentivirus |
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| LVP1011-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under the human CD68's promoter which expressed specifically in macrophages and macrophage-related cells. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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Flt1-RFP (GFP) Lentivirus |
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| LVP1014-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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Flt1-Luc (GFP) Lentivirus |
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| LVP1015-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under the human Flt-1's promoter which expressed specifically in endothelial cells and up-regulated in tumor vasculature. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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SPB-Rluc (GFP) Lentivirus |
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| LVP1028-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under human Surfactant protein B's promoter which selectively expressed in bronchiolar and alveolar epithelial cells of the lung. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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SYN1-RFP (GFP) Lentivirus |
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| LVP1030-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express RFP reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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SYN1-Luc (GFP) Lentivirus |
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| LVP1031-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Firefly luciferase reporter under human Synapsin 's promoter which used for neuron-specific high expression. This lentivirus also contain the GFP selection marker under the consitutiveRSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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aFP-Rluc (GFP) Lentivirus |
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| LVP1036-G | GenTarget | 1x107 IFU/ml x 200ul | EUR 346.5 |
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Description: Pre-made lentivirus express Renilla luciferase reporter under human alpha-fetoprotein 's promoter which used for the expression into hepatocellular carcinoma cells . This lentivirus also contain the GFP selection marker under the consitutive RSV promoter, provided in DMEM medium with 10% FBS and 60ug/ml of polybrene. |
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Self-Organizing Human Induced Pluripotent Stem Cell Hepatocyte 3D Organoids Inform the Biology of the Pleiotropic TRIB1 Gene
Institution of a physiologically related human hepatocyte-like cell system for in vitro translational analysis has been hampered by the restricted availability of cell fashions that precisely replicate human biology and the pathophysiology of human illness. Right here we report a strong, reproducible, and scalable protocol for the technology of hepatic organoids from human induced pluripotent stem cells (hiPSCs) utilizing quick publicity to nonengineered matrices.
These hepatic organoids comply with outlined levels of hepatic growth and specific greater ranges of early (hepatocyte nuclear issue 4A [HNF4A], prospero-related homeobox 1 [PROX1]) and mature hepatic and metabolic markers (albumin, asialoglycoprotein receptor 1 [ASGR1], CCAAT/enhancer binding protein α [C/EBPα]) than two-dimensional (2D) hepatocyte-like cells (HLCs) at day 20 of differentiation. We used this mannequin to discover the biology of the pleiotropic TRIB1 (Tribbles-1) gene related to numerous metabolic traits, together with nonalcoholic fatty liver illness and plasma lipids.
We used genome enhancing to delete the TRIB1 gene in hiPSCs and in contrast TRIB1-deleted iPSC-HLCs to isogenic iPSC-HLCs beneath each 2D tradition and three-dimensional (3D) organoid circumstances. Below typical 2D tradition circumstances, TRIB1-deficient HLCs confirmed maturation defects, with decreased expression of late-stage hepatic and lipogenesis markers.
In distinction, when cultured as 3D hepatic organoids, the differentiation defects had been rescued, and a transparent lipid-related phenotype was famous within the TRIB1-deficient induced pluripotent stem cell HLCs. Conclusion: This work helps the potential of genome-edited hiPSC-derived hepatic 3D organoids in exploring human hepatocyte biology, together with the practical interrogation of genes recognized by means of human genetic investigation.
Conditional reprogramming: subsequent expertise cellculture
Prolonged-term main custom of mammalian cells has been always robust attributable to unavoidable senescence. Customary methods for producing immortalized cell strains usually require manipulation of genome which leads to change of significant natural and genetic traits.
Not too way back, conditional reprogramming (CR) emerges as a novel subsequent expertise instrument for long-term custom of main epithelium cells derived from practically all origins with out alteration of genetic background of main cells.
CR co-cultures main cells with inactivated mouse 3T3-J2 fibroblasts throughout the presence of RHO-related protein kinase (ROCK) inhibitor Y-27632, enabling main cells to build up stem-like traits whereas retain their potential to fully differentiate.
With just some years’ development, CR reveals broad prospects in functions in diversified areas along with sickness modeling, regenerative medicine, drug evaluation, drug discovery along with precision medicine. This consider is thus to comprehensively summarize and assess current progress in understanding mechanism of CR and its broad functions, highlighting the value of CR in every main and translational researches and discussing the challenges confronted with CR.